Professional qPCR analysis in your browser.
No install. No cloud. No subscription.
Primer Validation ΔΔCt Expression Welch + ANOVA BH-FDR Correction PNG Export Offline
Get License — 9€ Try Demo →
qPCR Analyst v5.0
R² = 0.9987
GAPDH Curve
E = 98.3%
Efficiency
p = 0.0023
BH-FDR
2.87×
IL-6 Fold Change
Everything you need for rigorous qPCR analysis
Built by a scientist, for scientists. Follows MIQE guidelines.
📈
Primer Validation
Standard curve analysis with R², efficiency, slope. Automated QC against MIQE criteria (R² ≥ 0.99, E 90–110%). Outlier detection via median.
🧬
Expression Analysis
ΔΔCt method (Livak 2001). Multi-gene, multi-group with biological replicates. HKG stability scoring (geNorm M-values). Toggle reference genes on/off.
📊
Publication Statistics
Welch t-test (2 groups) and one-way ANOVA (3+ groups) with Benjamini-Hochberg FDR correction. Validated against scipy/R.
🎯
QC Pipetting Map
Visual plate-layout quality map. Each replicate colored by deviation from median — instantly spot pipetting errors across your entire experiment.
🖼️
Publication Figures
High-resolution PNG export (≈300 DPI) with composite stats panel. Group means ± SEM, p-values, sample dots. Ready for manuscript submission.
💾
Offline & Private
Single HTML file. Works without internet. Your data never leaves your computer. No account, no subscription, no cloud. Save/load sessions as JSON.
Simple pricing
One-time payment. Yours forever.
qPCR Analyst Pro
9€
One-time · Lifetime access · All future updates
☕ Buy on Ko-fi — 9€
You'll receive your license key instantly after purchase
Frequently asked questions
Everything you need to know.
How does it work after purchase?
You get a license key instantly via Ko-fi. Download qPCR Analyst from GitHub, open the HTML file, enter your key — done. Works in any browser, no install needed.
Does my data stay private?
Yes, completely. Everything runs locally in your browser. No data is sent to any server. Your experimental data never leaves your computer.
What's in the demo version?
The demo lets you explore the interface with pre-loaded example data. Export, custom data entry, and save/load are available in the full version.
Which browsers are supported?
Chrome, Firefox, Safari, Edge — any modern browser. Recommended: Chrome for best export quality.
Do I get future updates?
Yes. Your license key unlocks all future versions. Download updates from GitHub whenever a new version is released.
I have a question or found a bug.
Email us at qpcranalyst@gmail.com — we respond within 48h.