π¬
qPCR Analyst
Professional qPCR Analysis Tool
β Standard curve validation Β· β ΞΞCt analysis
β Welch t-test + ANOVA + BH-FDR Β· β PNG export
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Continue in demo mode
qPCR Analyst
πΎ Save
π Load
PRO
v5.0
π¬ Primer Validation
π Expression Analysis
π Paste from Spreadsheet
Paste tab-separated Ct values.
One row per sample
, columns = replicates per gene (in table order).
Cancel
Import
1
Standard Curve Setup
βΆ Demo
β Excel
Genes to validate
+ Add
No genes yetβ¦
Dilution factor
Points
Replicates
Starting conc.
β Generate Tables
1
Experiment Setup
βΆ Demo
All genes (comma-separated)
All genes measured, including housekeeping
Reference genes / Housekeeping (HKG)
Must be a subset of genes above β used for normalization (ΞCt)
Groups & biological replicates
(one per line: GroupName: n)
Samples auto-named: WT_1, WT_2, WT_3β¦
Control group
Technical replicates
β Generate Ct Table
2
Enter Ct Values
π Paste
βΆ Analyze
Outlier replicate (>0.5 Ct from mean) =
orange
β
click to exclude
(strikethrough)
π
Housekeeping Gene Stability
βΌ
M-value
= average expression variation relative to all other candidates.
M < 0.5 = stable
. V(i,j) = SD of ΞCt(iβj) across samples.
Stability ranking
Pairwise matrix V(i,j)
Reference Ct per sample
QC
Quality Report
βΌ
QC Score
Pipetting Precision β SD of technical replicates
SD <0.20 =
Excellent
Β· 0.20β0.50 =
Acceptable
Β· >0.50 =
Review
Ct per well
3
Results β 2
βΞΞCt
Log scale
β Excel
βΌ Overview β fold change
βΌ Per-sample results
βΌ Charts